Research Paper Advance Articles pp 23647—23667

Identification of the novel Np17 oncogene in human leukemia

Figure 4. Human leukemia cells express native Np17 protein and mRNA. (A) Western blot detection of native Np17 protein from a primary leukemia sample with Np17 antibody (α-Np17) and was blocked with recombinant Np17-6xHis protein (rNp17 protein). (B) Knock-down of Np17 with shRNA against Np17 significantly reduced nuclear Np17 protein level in THP-1 leukemia cells. (C) Western blot detection of native Np17 protein in human leukemia cell lines. (D) Western blot detection of native Np17 protein in primary human leukemia samples, the recombined Np17 protein of 293T cells was set as positive control. (E) Native Np17 protein was also detected in normal hematopoietic stem cells (CD34+ samples) but its level was lower as compared with AML samples (P1, P2). (F, G) detection of Np17 mRNA in human leukemia cell lines (F) and AML patient samples (G) with qRT-PCR.