Research Paper Volume 12, Issue 21 pp 21186—21201

Landscape of transcription and expression regulated by DNA methylation related to age of donor and cell passage in adipose-derived mesenchymal stem cells

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Figure 2. Characterization of ADSCs. (A) 3rd, 6th, and 10th passage Spindle-shaped ADSCs in young and old donors. Y-P3, Y-P6, Y-P10 represent the 3rd, 6th, and 10th passages of the young donor, respectively. O-P3, O-P6, O-P10 represent the 3rd, 6th, and 10th passages of the old donor, respectively. (B) Micrographs of 3rd generation ADSCs of young patients (up) and old patients (down) stained with Oil Red O. (C) Micrographs of 3rd generation ADSCs of young patients (up) and old patients (down) stained with Alizarin Red. (D) Micrographs of 3rd generation ADSCs of young patients (up) and old patients (down) stained with Alcian Blue.