Knockdown of NHP2 inhibits proliferation of PLC/PRF5 hepatoma cells with stable HBx overexpression or not. (A) The NHP2 mRNA was determined after PLC/PRF5 cells were transfected with shRNA-NC, shRNA-NHP2-1 or shRNA-NHP2-2. (B) The NHP2 mRNA was determined after HBx-overexpressed PLC/PRF5 cells were transfected with shRNA-NC, shRNA-NHP2-1 or shRNA-NHP2-2. (C) Representative western blot bands together with quantitative analysis for NHP2 expression in PLC/PRF5 cells of different groups. (D) Cell viability of PLC/PRF5 cells that stably overexpressed HBx or not in the absence or presence of NHP2 knockdown. (E) Representative colony formation assay of PLC/PRF5 cells from various groups. Ov: overexpression, NC: negative control, HBx: Hepatitis B virus X. *P<0.05, **P<0.01 and ***P<0.001.