Research Paper Volume 12, Issue 15 pp 15756—15770

MiR-483 induces senescence of human adipose-derived mesenchymal stem cells through IGF1 inhibition

Figure 5. Effects of miR-483-3p and IGF1 on senescence of hADSCs. (A) MiR-483-3p expression at early-passage (P7) and late-passage (P14) hADSCs was detected by RT-qPCR. (B) SA-β-gal staining of hADSCs (P14) transfected with 483-3p-M and corresponding control (10×; scale: 100μm). (C) SA-β-gal staining of hADSCs (P14) transfected with 483-3p-I and corresponding control (10×; scale: 100μm). (D) IGF1 expression in hADSCs at P7 and P14 was detected by RT-qPCR. (E, F) Efficiency of IGF1 inhibition was assayed by RT-qPCR and ELISA. (G) SA-β-gal staining of hADSCs (P14) transfected with si-CT or si-IGF1 (4×; scale: 100μm). (H) SA-β-gal staining of hADSCs (P14) co-transfected with 483-3p-I and si-IGF1 (10×; scale: 100μm).