Research Paper Volume 12, Issue 19 pp 19375—19398

HOXC10 promotes tumour metastasis by regulating the EMT-related gene Slug in ovarian cancer

Figure 2. HOXC10 accelerates OC cell migration. (I, J) Relative mRNA and protein expression levels of HOXC10 in 8910 cells transfected with the HOXC10 overexpression plasmid and empty vector. P<0.0001, P=0.0003. (K, L) Transwell and wound healing assays of Skov3 cells transfected with the HOXC10 overexpression plasmid and empty vector. P=0.0003, P<0.0001. Scale bars, 100 μm and 200 μm, respectively. (M) Fluorescence signal in 8910 cells transfected with the HOXC10 overexpression plasmid, HOXC10 NLS mutation plasmid and empty vector; all cells were stained with DAPI. Scale bars, 25 μm. (N, O) Transwell and wound healing assays of 8910 cells transfected with the HOXC10 overexpression plasmid, HOXC10 NLS mutation plasmid and empty vector. P=0.0005 and P=0.2381; P<0.0001 and P=0.0827. Scale bars, 100 μm and 200 μm, respectively.