Research Paper Volume 12, Issue 21 pp 21220—21235

Cyclooxygenase-2/sclerostin mediates TGF-β1-induced calcification in vascular smooth muscle cells and rats undergoing renal failure

Figure 6. Effects of meloxicam on adenine-induced renal failure in rats. (A) ELISA analysis results show the serum level of TGF-β1 in adenine-induced rat renal failure (n=10, "**" p<0.01 vs. control). (B) ELISA analysis results show the serum level of COX-2 in adenine-induced rat renal failure (n=10, "**" p<0.01 vs. control). (C) Western blot assay results show the protein level of TGF-β1 and COX-2 in the aorta from a rat; GAPDH was used as a loading control. (D) Von Kossa staining results show the calcification in aorta from adenine-induced rat renal failure, which were treated with preventive or therapeutic administration of meloxicam (Scale=100 μM). (E) H&E staining results show the pathological changes in adenine-induced rat renal failure, which were treated with preventive or therapeutic administration of meloxicam (Scale=50 μM). LP: low-dose prevention, HP: high-dose prevention, LT: little dose therapy, HT: high-dose therapy.