Research Paper Volume 12, Issue 20 pp 20366—20379

miR-140-3p inhibits bladder cancer cell proliferation and invasion by targeting FOXQ1

Figure 4. miR-140-3p suppresses proliferation and invasion of BCa cells by reducing FOXQ1 expression. (A, B), Western blot assay was performed to detect FOXQ1 expression. Approximately 50 ug of protein was loaded into each lane 2 to 4 days after transfection. (A) T24 cells were transfected with pWPI+NC, oeFOXQ1+NC, oeFOXQ1+miR-140-3p mimics or pWPI+miR-140-3p mimics. (B) UMUC3 cells were transfected with pLKO+NC, shFOXQ1+NC, shFOXQ1+inh-miR-140-3p or pLKO+ inh-miR-140-3p (C) An MTT rescue assay revealed that FOXQ1-increased cell proliferation could be reversed after adding miR-140-3p mimics to T24 cells. (D) An MTT rescue assay revealed that shFOXQ1-decreased cell proliferation could be reversed after adding miR-140-3p inhibitor to UMUC3 cells. (E) A transwell invasion assay revealed that FOXQ1-increased cell invasion could be reversed after adding miR-140-3p mimics to T24 cells. (F) A transwell invasion assay revealed that shFOXQ1-decreased cell invasion could be reversed after adding miR-140-3p inhibitor to UMUC3 cells. (CF) Each sample was run in triplicate and used in multiple experiments to determine the mean ± SD. *P < 0.05; **P < 0.01 compared to controls.