Research Paper Volume 12, Issue 20 pp 20380—20395

Rab1A promotes cancer metastasis and radioresistance through activating GSK-3β/Wnt/β-catenin signaling in nasopharyngeal carcinoma

Figure 3. Rab1A increases NPC cells metastasis, radioresistance and EMT phenotype. (A and B) Migration assay of 6-10B and S-26 cells with Rab1A overexpression or a control vector by transwell assay. Left, representative images of invading cells. Right, quantification of cell invasion data. (C) Survival curves of 6-10B and S-26 cells with Rab1A overexpression or a control vector created by the linear quadratic equation. Means ± SD (n=3) are shown in A, B and C. NS, no significant, *P <0.05 and **P < 0.01 versus control cells. (D) Western blotting assay to assess the expression of ZO-1, E-cadherin, N-cadherin and vimentin proteins in 6-10B and S-26 cells with Rab1A overexpression or 5-8F and CNE2 cells with Rab1A knockdown.