Research Paper Volume 12, Issue 22 pp 22582—22598

linc00968 inhibits the tumorigenesis and metastasis of lung adenocarcinoma via serving as a ceRNA against miR-9-5p and increasing CPEB3

Figure 4. miR-9-5p is highly expressed in LUAD tissues and exerts oncogenic roles in LUAD cell. (A) Relative expression levels of miR-9-5p in LUAD tissues were measured by qRT-PCR. (B, C) The expression levels of miR-9-5p in LUAD patients with different pathological stages and lymph node metastasis. **P<0.01 versus the I-II or patients with no lymph node metastasis. (D) Correlation analysis of linc00968 and miR-9-5p in 56 cases of LUAD tissues. (E) miR-9-5p expression in LUAD from TCGA. (F) Correlation analysis of linc00968 and miR-9-5p in TCGA. (G) Survival analysis of miR-9-5p in LUAD from TCGA. (H) miR-9-5p mimics was transfected A549 and H1975 cell. anti-miR-9-5p was transfected into HCC827 cell. The transfection efficiency was detected using qRT-PCR and immunofluorescence. (I, J) The colony formation and invasion abilities of LUAD cell transfected with miR-9-5p mimics or anti-miR-9-5p were measured by colony formation or Transwell invasion assay, respectively. **P<0.01 versus miR-Ctrl.