Research Paper Volume 12, Issue 22 pp 22599—22613

miR-25-3p promotes endothelial cell angiogenesis in aging mice via TULA-2/SYK/VEGFR-2 downregulation

Figure 1. MiR-25-3p expression in ECs: transfection with an miR-25-3p mimic or inhibitor regulates angiogenesis in vitro. (A) RT-qPCR analysis showed that the expression of miR-25-3p was downregulated in old endothelial cells (OECs) versus young endothelial cells (YECs) (n=3). (B) RT-qPCR analysis showed that the expression of miR-25-3p was upregulated in OECs with miR-25-3p mimics(n=3). (C) RT-qPCR analysis showed that the expression of miR-25-3p was downregulated in YECs with miR-25-3p inhibitor(n=3). (D) An EdU assay showed that mouse EC proliferation was increased by the transfection of miR-25-3p mimics into OECs, whereas the miR-25-3p inhibitor showed the opposite effect in YECs (n=5; Scale bar, 100 μm). (E) A wound-healing assay showed that miR-25-3p mimics promoted the migration ability of OECs, whereas the miR-25-3p inhibitor showed the opposite effect in YECs (n=4; scale bar, 200 μm). (F) Transwell assays showed that miR-25-3p mimics promoted the migration ability of OECs, whereas the miR-25-3p inhibitor showed the opposite effect in YECs (n=5; scale bar, 100 μm). (G) Tube formation determined on Matrigel showed that miR-25-3p mimics increased the total tube length of OECs, whereas the miR-25-3p inhibitor showed the opposite effect in YECs (n=4; Scale bar, 100 μm) (*P < 0.05, **P < 0.01).