Research Paper Volume 12, Issue 21 pp 21236—21252

IGF2 loss of imprinting enhances colorectal cancer stem cells pluripotency by promoting tumor autophagy

Figure 1. The association between IGF2 LOI and CSCs characteristics. (A) the correlation between IGF2 LOI and CD133 expression; (B) the mRNA expression of CSCs related genes in IGF2 LOI and MOI CRC cells; (C) the sphere formation assay in IGF2 LOI and MOI CSCs. After 7 days, IGF2 KD cells showed lower sphere formation efficiency than negative control or blank group (cells without any treatment) in IGF2 LOI CSCs(p<0.05). IGF2 OE cells showed higher sphere formation efficiency than negative control or blank group(p<0.05). Sphere forming efficiency = number of actual spheres/number of cells plated×100.