Research Paper Volume 12, Issue 21 pp 21236—21252

IGF2 loss of imprinting enhances colorectal cancer stem cells pluripotency by promoting tumor autophagy

Figure 3. The potential mechanism of IGF2 in CSCs regulation. (A) the expression levels of potential regulators downstream of IGF2. (B) the CO-IP results of IGF2 LOI CSCs with IGF2/IR-A/IGF1R antibodies; (C) the IR-A and IGF1R mRNA expression in TCGA and CRC patients; (D) the sphere formation efficiency of IGF2 LOI CSCs transfected with IR-A or IGF1R siRNA; (E) the changing of Akt, GSK3β expression in IGF2 LOI CSCs transfected with IR-A or IGF1R siRNA; (F) Typical pictures and dates of nude mice with tumorigenicity and tumors which were under different treatments.