Research Paper Volume 12, Issue 20 pp 20457—20470

Designing high affinity target-binding peptides to HLA-E: a key membrane antigen of multiple myeloma

Figure 7. Affinity of PEPTIDE 3 to HLA-E high expressed U266 cells. (A) HLA-E protein was not detected on U266 cells; pretreated U266 cells with leader peptide could induce HLA-E expression. (B) 5μg/ml PEPTIDE 3 interacted with HLA-E high expressed U266 cells; (C) 10μg/ml PEPTIDE 3 interacted with HLA-E high expressed U266 cells; (D) 25μg/ml PEPTIDE 3 interacted with HLA-E high expressed U266 cells; (E) 50μg/ml PEPTIDE 3 interacted with HLA-E high expressed U266 cells obviously; (F) 50μg/ml PEPTIDE 3 could not interacted with HLA-E blocking antibody pre-treated HLA-E high expressed U266 cells. The figures were representing ones and all repeated for three times.