Figure 1. The effects of SAM and L-dopa on the VEGFA expression in primary-HUVECs. (A and B) Western blot analysis of VEGFA protein level in primary-HUVECs treated with indicated concentrations of SAM or L-dopa for 24 h. (C) Western blot analysis of VEGFA protein level in primary-HUVECs treated with combined SAM and L-dopa for 24 h. (D and E) Real-time PCR analysis of VEGFA mRNA level after different concentrations of SAM or L-dopa treatment for 24 h. α-Tubulin was used as an internal normalization control. The quantitative results shown of three independent experiments are means ± SD. The asterisk (* or **) indicates a significant (p < 0.05 or p < 0.01, respectively) compared with control groups. NS indicated no significant difference.