Figure 2. SAM administration enhances the acetylation level of VEGFA in primary-HUVECs. (A) Prediction of acetylation sites in VEGFA protein sequence by PhosphoSitePlus website. (B) The primary cultured HUVECs cells were treated with 0.08 mM SAM for the 24 h, followed by Co-IP with antibody against VEGFA. Immunoprecipitates were immunoblotted with the indicated antibodies. (C) After treated with CHX (20 μg/ml) for the indicated times, protein levels of VEGFA were determined by western blot analyses of lysates from primary-HUVECs treated with indicated concentrations of SAM. (D) Quantification of VEGFA protein levels relative to α-Tubulin.