Figure 6. Increased salivary gland fibrosis and decreased salivary gland function. (A) Real time PCR of TNFα and IL-1β mRNA expression in the CON and OVX groups. (B) Fibrosis of submandibular gland detected by Masson and trichrome's staining. (C) Morphometric analysis of fibrosis in submandibular gland tissue in the CON and OVX groups. (D) Collagen type I expression of submandibular gland detected by immunohistochemistry. (E) Morphometric analysis of expression of collagen type I in the CON and OVX groups. (F) Saliva secretion in the CON and OVX groups. Two-way ANOVA test. *p<0.05, **p<0.01 and ***p<0.001. CON = control, OVX = ovariectomy, TNF = tumor necrosis factor, IL = interleukin.