Research Paper Volume 12, Issue 22 pp 22700—22718

Drug-selected population in melanoma A2058 cells as melanoma stem-like cells retained angiogenic features – the potential roles of heparan-sulfate binding ANGPTL4 protein

Figure 6. Effects of suppression of ANGPTL4 expression on cellular activities in drug-selected cells. (A) ANGPTL4 expression level was reduced by transfection of ANGPTL4-specific shRNA as examined by qPCR and flow cytometry. (n=3; data were mean ±SD; **, p < 0.01) (B) in vitro tube forming ability of drug-selected cells was abolished by the suppression of ANGPTL4 expression. (C) cell proliferation assay showed no difference in cell number between parental and drug-selected cells. (n=3; data were mean ±SD; ns: not significant with p = 0.08). (D) upon suppression of ANGPTL4 expression in drug-selected cells, the sensitivity toward carmustine was increased as indicated by less survival. (n=6; data were mean ±SD; **, p < 0.01; *, p < 0.05.). (E) sphere forming ability was abolished by suppression of ANGPTL4 expression. (F) transwell assay showed no difference in migrated cells between parental and drug-selected cells. (n=6; data were mean ±SD).