Research Paper Volume 12, Issue 22 pp 22719—22743

Gonadal white adipose tissue-derived exosomal MiR-222 promotes obesity-associated insulin resistance

Figure 6. The gWAT-derived exosomal miR-222 repress the expression of IRS1 protein in the liver and skeletal muscle tissues of HFD-fed obese model mice. (A, B) Western blot analysis shows the IRS1 protein levels in the (A) liver and (B) skeletal muscle tissues from the CD-fed and HFD-fed male mice. (C, D) Western blot analysis shows the IRS-1 protein levels in the (C) liver and (D) skeletal muscle tissues from the gWAT-lipectomized (gWL) and sham-operated (Sham) HFD mice. (E, F) Western blot analysis shows the IRS1 protein levels in the (E) livers and (F) skeletal muscle tissues in the 8-week old wild-type mice injected continuously for 7 days via the tail vein with CD-gWAT-Exos or HFD-gWAT-Exos. (G, H) Western blot analysis shows the IRS1 levels in the (G) liver and (H) skeletal muscle tissues in the HFD gWL mice injected continuously via the tail vein for 7 days with 293T-exosomes containing miR-NC or miR-222 mimics. (I, J) Western blot analysis shows the IRS1 levels in the (I) liver and (J) skeletal muscle tissues of the wild-type mice continuously injected for 7 days with the HFD-gWAT-Exos plus 293T-exosomes (containing antagomiR-222 or antagomiR-NC). Note: CD-gWAT-Exos: exosomes secreted by the gWAT of CD mice; HFD-gWAT-Exos: exosomes secreted by the gWAT of HFD mice; The IRS1 protein levels were normalized to α-tubulin levels; The data are presented as the means ± SE; * P < 0.05, ** P < 0.01, *** P < 0.001.