Research Paper Advance Articles pp 23684—23697

Hypoxia induces pulmonary artery smooth muscle dysfunction through mitochondrial fragmentation-mediated endoplasmic reticulum stress

Figure 4. Drp1 overexpression induced ER stress. (A) Drp1 was overexpressed by adenovirus in PASMCs. Twenty micrograms of protein was loaded for each lane. (B) Overexpression of Drp1 induced mitochondrial fragmentation as detected by mitoTracker. Scale bar, 20 μm. (C) Overexpression of Drp1 increased mitochondrial ROS as detected by mitoSOX. Scale bar, 100 μm. (D) Drp1 overexpression increased CHOP expression in PASMCs. Twenty micrograms of protein was loaded for each lane. (E) Drp1 overexpression decreased PASMCs function as evidenced by decreased PE/SNP-induced MLC phosphorylation/dephosphorylation in PASMCs. Twenty micrograms of protein was loaded in each lane. *, p < 0.05, **, p < 0.01. n = 8.