Research Paper Volume 12, Issue 21 pp 21423—21445

Smyd3-PARP16 axis accelerates unfolded protein response and vascular aging

Figure 1. PARP16 is involved in Ang II-induced endoplasmic reticulum (ER) and endothelial cells senescence. (A) ER markers were upregulated after Ang II stimulation in RAECs. At 0, 3, 6, 12, 24 and 48 h after Ang II (2 μM) administration, cells extracts were collected for determining the protein levels. Data shown are representative of data from at least three different replicates. (B) qRT-PCR analysis of the UPR target genes (Hspa5, Ddit3, Atf4) after Ang II stimulation for indicated time in RAECs. Data shown are technically representative of data from at least three different replicates; ###p < 0.001 vs. 0 h. (C) PARP16 and senescence-associated marker p21 were upregulated after Ang II stimulation in RAECs. At 0, 3, 6, 12, 24 and 48 h after Ang II (2 μM) administration, cells extracts were collected for determining the protein levels. Data shown are representative of data from at least three different replicates. (D) Immunofluorescence double staining of PARP16 and p53, and quantitative analysis in RAECs upon Ang II treatment, ###p < 0.001 vs. control. ‘DAPI’ represents DAPI staining of nuclei (DNA) throughout the manuscript.