Research Paper Volume 12, Issue 21 pp 21423—21445

Smyd3-PARP16 axis accelerates unfolded protein response and vascular aging

Figure 2. PARP16 overexpression promotes RAECs senescence and endoplasmic reticulum. RAECs were transfected with lentivirus-mediated PARP16 cDNA (PARP16 OE) for 72 h, Parp16, p21, p53, Il-6, Vcam-1, Inos mRNA level were confirmed by qRT-PCR. Data shown are representative of data from at least three different replicates; ###p < 0.001 vs. control. (A); cell lysates were immunoblotted with antibody against PARP16, p53, p21 and VCAM-1. GAPDH serves as internal control (B); SA-β-Gal staining (C) and EdU incorporation assay (D) of RAECs upon overexpression of PARP16, ##p < 0.01, ###p < 0.001 vs. control, n=5; (E) p-PERK, p-eIF2α, Bip and Spliced XBP-1 level were determined in PARP16 overexpressing cells. Data shown are representative of data from at least three different replicates.