Research Paper Volume 12, Issue 20 pp 20493—20511

RNA-seq reveals the diverse effects of substrate stiffness on epidermal ovarian cancer cells

Figure 1. Cell morphology closely correlate with substrate rigidity. (A) Typical DIC images of SKOV-3 cultured on polyacrylamide hydrogels with different substrate rigidity. SKOV-3 cells were plated on collage I-coated 1 kPa, 6 kPa and 60 kPa hydrogels, and then images were taken at 12 h, 24 h or 48 h. (B) SKOV-3 cells plated on collage I-coated 1 kPa, 6 kPa and 60 kPa hydrogels for 24 h were fixed and stained with CellTracker fluorescent probes (left) and DAPI (middle) to visualize cytoplasm and nucleus respectively. Scale bar - 40 μm.