Research Paper Volume 12, Issue 22 pp 22759—22775

NEAT1/miR-200b-3p/SMAD2 axis promotes progression of melanoma

Figure 1. miR-200b-3p was down-regulated in malignant melanoma and associated with poor overall survival. (A) GSE35579, GSE143231, GSE143777, GSE138710, GSE138412, GSE117666 profiles were analyzed to screen melanoma-related miRNAs. (B) Levels of miR-200b-3p between benign nevus (n=11) and malignant melanoma (n=20) tissues were analyzed according to GSE35579. (C) Levels of miR-200b-3p of clinic samples between benign nevus (n=18) and malignant melanoma tissues (n=18) were measured by RT-qPCR. (D) Levels of miR-200b-3p in melanoma cells lines (A375, A875 and M14) compared to benign epithelial cell, HEMa-LP, by RT-qPCR. (E) Kaplan–Meier survival curves for melanoma patients with high (n=56) or low (n=381) miR-200b-3p levels. (F) Kaplan–Meier survival curves for mesothelioma patients with high (n=43) or low (n=42) miR-200b-3p levels.