Research Paper Volume 12, Issue 20 pp 20523—20539

Exosomal long non-coding RNA UCA1 functions as growth inhibitor in esophageal cancer

Figure 3. Effect of overexpression of UCA1 on esophageal cancer cells. (A) Expression of UCA1 in esophageal cancer cell lines EC18 and Kyse140 transfected with UCA1-expressing plasmids (vector-UCA1) or negative control plasmids (vector-NC). (B, C) Cell viability of EC18 and Kyse140 cells transfected with UCA1-expressing plasmids (vector-UCA1) or negative control plasmids (vector-NC). Scale bar = 50 nm. (D) Invasive ability of EC18 and Kyse140 cells transfected with UCA1-expressing plasmids (vector-UCA1) or negative control plasmids (vector-NC). Scale bar = 50 nm. (E) Migratory ability of EC18 and Kyse140 cells transfected with UCA1-expressing plasmids (vector-UCA1) or negative control plasmids (vector-NC). Scale bar = 50 nm. (F) Colony formation ability of EC18 and Kyse140 cells transfected with UCA1-expressing plasmids (vector-UCA1) or negative control plasmids (vector-NC). (*) denotes the difference between groups (P<0.05). Each experiment had a minimum of three replicates per treatment group.