Research Paper Volume 12, Issue 20 pp 20523—20539

Exosomal long non-coding RNA UCA1 functions as growth inhibitor in esophageal cancer

Figure 6. Effect of exosomal UCA1 on tumor growth in vivo. (A) Representative images of nude mice treated with EC18 cells transfected with UCA1-expressing vector (UCA1-vector) or negative control vector (Control), or exosomes derived from NEEC cells transfected with UCA1-expressing plasmids (UCA1-Exo) or negative control plasmids (Control- Exo). (B) Tumor volumes of mice treated with EC18 cells transfected with UCA1-expressing vector (UCA1-vector) or negative control vector (Control), or exosomes derived from NEEC cells transfected with UCA1-expressing plasmids (UCA1-Exo) or negative control plasmids (Control- Exo). (C, D) Representative images of tumor tissues and tumor weights of mice treated with EC18 cells transfected with UCA1-expressing vector (UCA1-vector) or negative control vector (Control), or exosomes derived from NEEC cells transfected with UCA1-expressing plasmids (UCA1-Exo) or negative control plasmids (Control- Exo). (E) H&E staining assay for Ki67 expression in tumor tissues. (F) Immunohistochemistry assay for Ki67 expression in tumor tissues. (G) Putative binding site of miRNA-613 in UCA1. (H) Luciferase reporter assay. (I) Expression of miRNA-613 in esophageal cancer tissues (Tumor) and paired normal tissues (Control). (J) Pearson’s correlation between expressions of miRNA-613 and UCA1 in esophageal cancer tissues. (*) denotes the difference between groups (P<0.05). Each experiment had a minimum of three replicates per treatment group.