Research Paper Volume 12, Issue 21 pp 21469—21480

The protective effects of memantine against inflammation and impairment of endothelial tube formation induced by oxygen-glucose deprivation/reperfusion

Figure 4. Memantine suppressed oxygen-glucose deprivation/reperfusion-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). Cells were treated with memantine (5, 10 μM) for 6 h, followed by exposure to oxygen-glucose deprivation (6 h)/reperfusion (24 h) (OGD/R). (A) Intracellular ROS was measured by dihydroethidium (DHE); (B) mRNA of NOX-4 as measured by real-time PCR; (C) Protein of NOX-4 as measured by western blot analysis (####, P<0.0001 vs. vehicle group; ***, *****, P<0.001, 0.0001 vs OGD/R group).