Research Paper Volume 12, Issue 21 pp 21674—21686

Circular RNA circSEMA5A promotes bladder cancer progression by upregulating ENO1 and SEMA5A expression

Figure 1. circSEMA5A is upregulated in BC. (A) Diagram showed the circSEMA5A derived from exons 3 and 4 of SEMA5A gene. The back-splicing junction site of circSEMA5A was confirmed by PCR amplification and followed by Sanger sequencing. (B) The relative expression of circSEMA5A was detected by qRT-PCR in BC tissues and paired normal bladder tissues. (C) The relative expression of circSEMA5A was detected by qRT-PCR in BC cell lines and a normal human uroepithelial cell line. (D and E) The transfection efficiencies were verified by qRT-PCR when T24 cells were transfected with circSEMA5A overexpression plasmids and UM-UC-3 cells were transfected with circSEMA5A siRNAs. Data are presented as mean ± SD. *P < 0.05.