Research Paper Volume 12, Issue 21 pp 21674—21686

Circular RNA circSEMA5A promotes bladder cancer progression by upregulating ENO1 and SEMA5A expression

Figure 5. CircSEMA5A facilitates the oncogenic process of BC cells via miR-330-5p/ENO1. (AH) T24 cells were transfected with circSEMA5A overexpression plasmids, miR-330-5p mimics and ENO1 overexpression plasmids; UM-UC-3 cells were transfected with circSEMA5A siRNAs, miR-330-5p inhibitors and ENO1 siRNAs. (A and B) Cell proliferation was measured by EdU assays; (C and D) cell apoptosis was detected by flow cytometry; (E and F) cell migration was assessed using wound-healing assays; (G and H) cell invasion was evaluated transwell assays. Data are presented as mean ± SD. *P < 0.05.