Research Paper Advance Articles pp 23778—23794

Colon cancer combined with obesity indicates improved survival- research on relevant mechanism

Figure 3. Obesity was associated with repressed hypoxia in colon cancer via inhibiting miR-210. (A) Differentially expressed miRNAs (DEMs) between obesity and normal groups. (B) The relationship of OS and miR-210 (divided by median of expression) performed by Kaplan-Meier analysis. (C) KEGG pathway analysis of miR-210-targeted genes (predicted by TargetScan database). (D) GO analysis of miR-210-targeted genes. (E) Hypoxia score of obesity and normal groups, derived from three different hypoxia scoring systems (Ragnum, Winter, Buffa hypoxia system). (F) The correlation of miR-210 and 3 different hypoxia scores analyzed by Pearson Correlation Coefficient. (G) Differentially expressed proteins between obesity and normal groups derived from Reverse Phase Protein Arrays (RPPA) data. (H) The invasion and migration ability of HCT-116 cells under hypoxia and normoxia. (I) The proliferation ability of HCT-116 cells under hypoxia and normoxia. (J) The expression of hypoxia associated genes of HCT-116 cells under hypoxia and normoxia. (*p<0.05, **p<0.01, ***p<0.001).