Research Paper Volume 12, Issue 21 pp 21706—21729

Puerarin alleviates osteoporosis in the ovariectomy-induced mice by suppressing osteoclastogenesis via inhibition of TRAF6/ROS-dependent MAPK/NF-κB signaling pathways

Figure 1. Puerarin protects against bone mass loss in the in vivo OVX-induced osteoporosis model mice. (A) Representative 3D- μCT images of the femur bone in the sham, OVX+vehicle, and OVX+puerarin group mice. (BG) Comparative analysis of the bone structural parameters such as bone mass density (BMD in g/mm3), ratio of bone volume to total volume (BV/TV in %), ratio of bone surface to bone volume (BS/BV in 1/mm), ratio of bone surface to total volume (BS/TV in 1/mm), connectivity density (Conn.Dn in 1/mm) and trabecular number (Tb.N in 1/mm2)in the sham, OVX+vehicle, and OVX+puerarin group mice (n=5 per group). Note: **P <0.01 vs. OVX+vehicle group. (H) Representative H&E stained femur bone sections of the sham, OVX+vehicle, and OVX+puerarin group mice (n=5 per group). (IJ) The analysis of the bone parameters, BV/TV and BS based on the H&E staining sections of the femur bone from the sham, OVX+vehicle, and OVX+puerarin group mice (n=5 per group). Note: **P <0.01 vs. the OVX+vehicle group.