Research Paper Volume 12, Issue 21 pp 21706—21729

Puerarin alleviates osteoporosis in the ovariectomy-induced mice by suppressing osteoclastogenesis via inhibition of TRAF6/ROS-dependent MAPK/NF-κB signaling pathways

Figure 2. Puerarin treatment reduces the numbers of osteoclasts in the bone tissue of OVX-induced osteoporosis model mice. (A, B) Representative images show the tartrate-resistant acid phosphatase (TRAcP) activity staining in the bone sections from the sham, OVX+vehicle, and OVX+puerarin mice. Also shown is the estimation of the osteoclast surface relative to the bone surface (Oc.S/BS) in the femur bones of the sham, OVX+vehicle, and OVX+puerarin group mice (n=5 per group). Note: **P <0.01 vs. the OVX+vehicle group. (CE) Representative images show the IHC staining of the femur bone sections from the sham, OVX+vehicle, and OVX+puerarin mice with antibodies against bone resorption markers, MMP9 and NFATc1. Also shown is the quantitative analysis of MMP9- and NFATc1-postive cells in the femur bone sections from the sham, OVX+vehicle, and OVX+puerarin mice (n=5 per group). Note: **P <0.01 vs. the OVX+vehicle group.