Research Paper Volume 13, Issue 2 pp 1883—1897

CD36 upregulates DEK transcription and promotes cell migration and invasion via GSK-3β/β-catenin-mediated epithelial-to-mesenchymal transition in gastric cancer

CD36 promotes c-Myc-dependent DEK transcription in GC cells. (A) Western blot analysis in SNU-216 cells transfected with NC/siCD36. *p  (B) Schematic diagram of the DEK gene promoter; the c-Myc-binding site is highlighted. (C) Analysis of DEK mRNA expression following siRNA-mediated c-Myc knockdown (sic-Myc) in SNU-216 cells. *p D) Dual luciferase assay results from SNU-216 cells co-transfected with firefly luciferase constructs (pGL4.10-DEK Promoter-WT or pGL4.10-DEK Promoter-Mut) and pcDNA3.1-c-Myc. Left: firefly luciferase constructs; right: relative luciferase activity. *p All data are presented as the mean ± SD.

Figure 5. CD36 promotes c-Myc-dependent DEK transcription in GC cells. (A) Western blot analysis in SNU-216 cells transfected with NC/siCD36. *p < 0.05 vs. NC group. GAPDH was used as endogenous control. (B) Schematic diagram of the DEK gene promoter; the c-Myc-binding site is highlighted. (C) Analysis of DEK mRNA expression following siRNA-mediated c-Myc knockdown (sic-Myc) in SNU-216 cells. *p < 0.05 vs. NC group. (D) Dual luciferase assay results from SNU-216 cells co-transfected with firefly luciferase constructs (pGL4.10-DEK Promoter-WT or pGL4.10-DEK Promoter-Mut) and pcDNA3.1-c-Myc. Left: firefly luciferase constructs; right: relative luciferase activity. *p < 0.05 vs. GL4.10-DEK Promoter-WT + pcDNA3.1 group. All data are presented as the mean ± SD.