Research Paper Volume 12, Issue 21 pp 21874—21889

Integrated analysis of transcriptomic and metabolomic data demonstrates the significant role of pyruvate carboxylase in the progression of ovarian cancer

Figure 7. Metabolomics data analysis for the identification of potential metabolic pathways regulated by PC. (A) Heatmap of differential metabolites in shPC vs. shNC groups in positive mode and negative mode. A bidirectional clustering analysis heat map was used to visualize metabolite levels in shPC vs. shNC samples under positive (left) and negative modes (right). The horizontal and vertical axes represent the samples and metabolites, respectively. Green represents down-regulated levels while red refers to up-regulated levels. (B) Volcano map of differential metabolites under positive (left) and negative (right) modes in shPC vs. shNC samples. The points in pink, blue, and grey refer to metabolites with up, down, and normal regulated levels, respectively. The dot size represents the VIP value. (C) Functional enrichment analysis for differential metabolites. The vertical and horizontal axes represent pathway categories and count number, respectively. The dot size represents the metabolite ratio of pathway enrichment. The color changes from blue to red refer to decreasing p-values. A dot with a smaller p-value represents a more significant difference for the pathway category. (D) Integrated analysis of transcriptomic and metabolomics data to identify crucial pathways regulated by PC.