Figure 4. CALR knockdown inhibits G1 to S-phase cell cycle transition in NKTCL cell lines. (A, B) Flow cytometry analysis shows the proportions of G0/G1 phase and S-phase cells in the control and CALR-knockdown SNK6 and SNT8 cell lines. The cells were stained with PI. (C) Western blot analysis shows the expression of cell cycle regulatory proteins, P27, CDK2 and Cyclin E2 in the control and CALR-knockdown SNK6 and SNT8 cell lines. Note: All experiments were performed in triplicates; P values were analyzed using ANOVA; **** P < 0.0001, *** P < 0.001.