Figure 4. Knockdown of hsa_circ_0001869 decreased the invasion ability of NSCLC in vitro and in vivo. (A–C) Cell migration and invasion were determined in A549 and PC9 cells using the Transwell® assay. n = 3. Data are expressed as mean ± SD. ***P < 0.001 vs. NC. (D) Live imaging of the effects of hsa_circ_0001869 on metastasis of A549 cells 30 days after intravenous tail injection. (E) Number of lung metastatic nodules by group. n = 6. Data are expressed as mean ± SD. ***P < 0.001 vs. NC.