Figure 10. SEMA4C silencing decreases the expression of EMT markers in the LoVo cells. (A) QRT-PCR analysis shows the relative mRNA expression of EMT-related genes in the control and SEMA4C-silenced LoVo cells. ***, P < 0.001; **, P < 0.01; *, P < 0.05. (B, C) Representative immunofluorescence images (Scale bars=25 μm) show the levels of E-cadherin (red) (B) and Vimentin (green) (C) proteins in the control and SEMA4C-silenced LoVo cells. The nuclei are stained with Hoechst (blue).