Research Paper Volume 12, Issue 21 pp 21992—22018

High SEMA4C expression promotes the epithelial-mesenchymal transition and predicts poor prognosis in colorectal carcinoma

Figure 9. SEMA4C silencing decreases proliferation, migration, and invasion of the LoVo cells. (A) QRT-PCR analysis shows the SEMA4C mRNA levels in the NC-siRNA- or SEMA4C-siRNA-transfected LoVo cells (human colon adenocarcinoma cell line). (B) CCK-8 assay results show the viability of control and SEMA4C-silenced LoVo cells. (C) Wound-healing assay results show the migration rates of control and SEMA4C-silenced LoVo cells. Note: Scale bars=100 μm. (D) Transwell invasion assay results show the invasiveness of control and SEMA4C-silenced LoVo cells. Note: Scale bars=10 μm. H indicates hour. ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, not significant.