Research Paper Volume 12, Issue 21 pp 22019—22045

The nuclear and cytoplasmic roles of miR-320 in non-alcoholic fatty liver disease

Figure 3. MiR-320 overexpression increased liver lipid content in differently treated mice. (A) MiR-320 levels in STZ-treated C57BL/6 mice, HFD-treated C57BL/6 mice, and db/db mice liver were determined by quantitative real-time PCR (n=6). (B) Cytoplasmic and nuclear miR-320 levels in normal mice liver were detected by cell fractionation followed by RT-qPCR. GAPDH mRNA and U6 RNA were served as cytoplasmic and nuclear markers. MiR-320 was similarly expressed in nucleus and cytoplasm (n=3, *p<0.05). (C, D) Cytoplasmic and nuclear miR-320 levels in STZ-treated C57BL/6 mice (B), HFD-treated C57BL/6 mice (C), and db/db mice (D) liver were determined using cell fractionation followed by quantitative real-time PCR (n=3, *p<0.05).