Research Paper Volume 12, Issue 21 pp 22046—22058

miR-205 inhibits the development of hypertrophic scars by targeting THBS1

Figure 2. miR-205 inhibits growth and migration of hypertrophic scar fibroblasts. (A, B) The viability of cells deprived miR-205 or expressing miR-205 was measured by MTT and colony formation assay. **P < 0.05, vs. control. ##P < 0.05, vs. scramble. (C, D) The apoptosis of cells deprived miR-205 or expressing miR-205 was measured by Hoechst 33258 assay and Annexin V-PI assay. **P < 0.05, vs. control. ##P < 0.05, vs. scramble. (E) The migration of cells deprived miR-205 or expressing miR-205 was measured by Transwell assay. **P < 0.05, vs. control. ##P < 0.05, vs. scramble. Results show the mean ± SD of three independent experiments. (F, G) THBS1, TGF-β1, α-SMA, Col1, and Cleavage Caspase 3 expression was detected. Cells were transfected with control, scramble, miR-205, and miR-205 inhibitor, respectively. **P < 0.05, vs. control.