Figure 4. PI3K inhibitors wortmannin and LY294002 reversed the increase in Nrf2 nuclear translocation and GSK-3β phosphorylation and prevented the protective effect induced by APG. (A) Comprehensive photograph of p-GSK-3β (Ser-9), total GSK-3β, and nuclear Nrf2 expression and the corresponding β-actin and histone-3 bands. (B) Effect of wortmannin and LY294002 on the increased Nrf2 nuclear translocation mediated by APG. (C) Effect of wortmannin and LY294002 on the increased phosphorylation of GSK-3β induced by APG. (D) Effect of wortmannin and LY294002 on the plasma lactate dehydrogenase (LDH) release level in primary cortical neuron cultures treated with APG. (E) Effect of wortmannin and LY294002 on cell viability in primary cortical neuron cultures with APG treatment. n = 5 per group. * P < 0.05, ** P < 0.01, *** P < 0.001.