Research Paper Volume 12, Issue 21 pp 22112—22121

Autophagy modulates mesenchymal-to-endothelial transition via p53

Figure 3. MEndT was modulated by autophagy by regulating the expression of nuclear p53. Cells were treated with TGF-β and EBSS as described above. (A, B) p53 expression in the cytoplasm and nucleus was evaluated by Western blot. (C, D) Cells were infected with lv-p53 for 24 h, and infection was assessed by the expression of p53. (E, F) p53 expression in cells treated with lv-p53-shRNA. (G, H) After overexpressing p53, cells were treated by the method described above, CD31, VE-cadherin, Vimentin and α-SMA levels were analyzed by immunoblot. (I, J) CD31, VE-cadherin, Vimentin and α-SMA expression in cells treated with lv-p53-shRNA using the method described above. Bar graphs represent data from three independent experiments and data represent the means±SEM. Unpaired T test (*P<0.05) was used to compare the significances between two groups.