Research Paper Volume 12, Issue 22 pp 23096—23113

Protective effect of DLX6-AS1 silencing against cerebral ischemia/reperfusion induced impairments

Figure 5. miR-149-3p may be a target of DLX6-AS1. (A) Predicted binding miRNAs of DLX6-AS1 derived from 3 databases. (B) Luciferase assay results of DLX-AS1 wild type (WT) and mutant (MUT) construct binding with mmu-miR-149-3p. (C) Effects of a miR-149-3p mimic on apoptosis induced by OGD/R, as detected by TUNEL staining. (D) Effects of a miR-149-3p mimic on caspase-3 expression induced by OGD/R in N2a cells detected by western blotting. (E) Effects of a miR-149-3p inhibitor on apoptosis induced by OGD/R, as detected by TUNEL staining. (F) Effects of a miR-149-3p inhibitor on caspase-3 expression induced by OGD/R in N2a cells detected by western blotting. Values represent the mean ± SEM (n = 3 in each group). *P < 0.05, ***P < 0.001 vs NEG, **P < 0.01 vs Ctrl; +P < 0.05 vs OGD 3 h/R 24h + NEG-mimic, +++P < 0.001 vs OGD 3 h/R 24 h + NEG; #P < 0.05, ##P < 0.01 vs OGD 3 h/R 24 h + DLX6-AS1-shRNA.