Research Paper Advance Articles pp 24009—24022

Extracellular vesicle derived miR-544 downregulates expression of tumor suppressor promyelocytic leukemia zinc finger resulting in increased peritoneal metastasis in gastric cancer

Figure 4. miR-544 from GC-derived EVs of PM patients reduces PLZF expression in HMrSV5 cells. (A) mRNA and (B) protein expression of PLZF in EVs isolated from GC patients with and without PM. (n=3, Student’s t-test for A, B) (C) RT-PCR demonstrating increased levels of miR-223-3p, miR-21-5p, and miR-544-5p in EVs isolated from GC patients with PM. (D) RT-PCR of miR-544 in HMrSV5 cells transfected with miR-544 mimics or miR-544 inhibitors. (E) Western blotting of PLZF in HMrSV5 cells transfected with miR-544 mimics or miR-544 inhibitors. (n=3, one way ANOVA for C, D, E).