Expression of Concern
This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 12, Issue 23 pp 24009—24022
Extracellular vesicle derived miR-544 downregulates expression of tumor suppressor promyelocytic leukemia zinc finger resulting in increased peritoneal metastasis in gastric cancer
Figure 4. miR-544 from GC-derived EVs of PM patients reduces PLZF expression in HMrSV5 cells. (A) mRNA and (B) protein expression of PLZF in EVs isolated from GC patients with and without PM. (n=3, Student’s t-test for A, B) (C) RT-PCR demonstrating increased levels of miR-223-3p, miR-21-5p, and miR-544-5p in EVs isolated from GC patients with PM. (D) RT-PCR of miR-544 in HMrSV5 cells transfected with miR-544 mimics or miR-544 inhibitors. (E) Western blotting of PLZF in HMrSV5 cells transfected with miR-544 mimics or miR-544 inhibitors. (n=3, one way ANOVA for C, D, E).