Research Paper Advance Articles pp 24009—24022

Extracellular vesicle derived miR-544 downregulates expression of tumor suppressor promyelocytic leukemia zinc finger resulting in increased peritoneal metastasis in gastric cancer

Figure 5. miR-544 is transferred from GS-derived EVs to peritoneal cells. (A) RT-PCR of miR-544 in GC tissues. (B) RT-PCR of miR-544 in EVs isolated from GC patients with and without PM. (n=68 for GC patients without PM, n=65 for GC patients with PM, two-tailed unpaired student’s t-tests) (C) RT-PCR of miR-544 in GC cell lines MGC803, BGC823, MKN45, HGC27, and SGC7901. (D) RT-PCR of miR-544 in EVs isolated from GC and GES-1 cells. (E) PKH67-labeled EVs in HMrSV5 cells (bar represents 10 μm). (F) PLZF expression in HMrSV5 cells co-cultured with MGC-803-and GES-1-derived EVs. (n=3, one way ANOVA for C, D, E and F).