Research Paper Advance Articles pp 24009—24022

Extracellular vesicle derived miR-544 downregulates expression of tumor suppressor promyelocytic leukemia zinc finger resulting in increased peritoneal metastasis in gastric cancer

Figure 6. miR-544 inhibition increases PLZF expression in HMrSV5 cells. (A) RT-PCR of miR-544 in EVs isolated from MGC803 cells transfected with Len-miR-544-inhibitor or control Len-miR-544-NC. (B) Western blotting of PLZF in MGC803 cells transfected with Len-miR-544-inhibitor or Len-miR-544-NC. (C) RT-PCR of exosomal miR-544 derived from MGC803 cells transfected with Len-miR-544-inhibitor or Len-miR-544-NC. (D) PLZF mRNA in MGC803 cells transfected with Len-miR-544-inhibitor or Len-miR-544-NC. (E) RT-PCR of miR-544 in Len-miR-544-inhibitor group and Len-miR-544-NC group in HMrSV5 cells co-cultured with EVs derived from MGC803 Len-miR-544-NC or Len-miR-544-inhibitor. (F) PLZF expression in EVs isolated from MGC803 cells transfected with Len-miR-544-inhibitor or Len-miR-544-NC. (n=3, Student’s t-test)