Research Paper Volume 12, Issue 21 pp 22174—22198

Nucleolar stress induces a senescence-like phenotype in smooth muscle cells and promotes development of vascular degeneration

Figure 1. Altered expressions of components of the RNA polymerase I (Pol I) machinery in animal and human abdominal aortic aneurysm (AAA) tissues. (A) Real-time PCR results showing time-dependent changes of various Pol I components in wild type mouse abdominal aortas with CaCl2/phosphate-induced AAA (n = 3 - 6). (B) Real-time PCR results showing time-dependent changes of various Pol I components in apolipoprotein E (ApoE)-/- mouse abdominal aortas with angiotensin II-induced AAA (n = 4 - 7). (C) Real-time PCR results showing changes in the expression of various Pol I components and pre-rRNA in human AAA tissues as compared to normal aortas (Con) (n = 4 - 6). The level of 45S pre-rRNA was normalized with the total 18S mature rRNA. For other genes, GAPDH or β-actin was used as the housekeeping gene. Dot blot-combined bar graphs represented mean ± S.D. * P < 0.05, ** P < 0.01, *** P < 0.001, unpaired t-test or one-way ANOVA as appropriate. The n number represents biological replications (same for all figures). ns, no significance.