Research Paper Advance Articles pp 24033—24056

lncRNA DLEU2 acts as a miR-181a sponge to regulate SEPP1 and inhibit skeletal muscle differentiation and regeneration

Figure 5. (A) mRNA expression levels of SEPP1 and myogenic markers (MyoD and MyoG) in C2C12 cells transfected with DLEU2 as detected by RT-PCR assay. (B) Proliferation of C2C12 cells following DLEU2 overexpression. (C) mRNA expression levels of SEPP1, MyoD and MyoG in C2C12 cells transfected with DLEU2 shRNA as detected by RT-PCR assay. (D) Proliferation of C2C12 cells following transfection with DLEU2 shRNA. (E) C2C12 myoblasts were treated with DLEU2 or shRNA-1/2. EDU assays demonstrated that treatment with DLEU2 reduced cell proliferation and the level of EDU-positive C2C12 cells. Quantification of relative ratio of Edu+ C2C12 cells. Data are presented as the mean ± SD (n = 3). Versus control or NC, ** p < 0.01, ***p <0.005. (F–H) Protein expression levels of SEPP1, MyoD and MyoG in C2C12 cells transfected with DLEU2 or DLEU2 shRNA as detected by western blot assay.