Research Paper Volume 12, Issue 22 pp 23233—23250

NEAT1 regulates microtubule stabilization via FZD3/GSK3β/P-tau pathway in SH-SY5Y cells and APP/PS1 mice

Figure 1. NEAT1 silencing induces de-polymerization of microtubules (MTs). (A) The expression of NEAT1 in the hippocampi of AD patients with different braak stage and normal persons was analyzed in GSE84422. (B) NEAT1 analysis in the hippocampi of 3.5-month-old AD mice and Wild Type. (C) The NEAT1 mRNA level was measured by quantitative PCR in shNEAT1v2 cells and shCtrl cells. (D) NEAT1 mRNA level was detected by quantitative PCR in shNEAT1-Mus and shCtrl transfected murine neurons. (E) Immunofluorescence staining of α-tubulin (red) in shNEAT1-Mus and shCtrl transfected murine neurons. (F) Morphological changes of murine neurons were observed under light microscope after NEAT1 knockdown. (G) Immunofluorescence analysis of α-tubulin (red) in shNEAT1v2 cells and shCtrl cells. DAPI (blue) was used to stain the nuclei. Scale bars, 20μm. Image J software was used to analyze the cell dendritic length (mean ± s.d, *P < 0.05, **P < 0.01, ***P < 0.001, Student 2-tailed t test).