Research Paper Volume 12, Issue 22 pp 23326—23336

The DLG1-AS1/miR-497/YAP1 axis regulates papillary thyroid cancer progression

Figure 5. DLG1-AS1 regulates YAP1, the target gene of miR-497 in PTC cells. (A, B) The expression of YAP1 was examined in TPC-1 cells transfected with sh-NC, sh-DLG1-AS1, and sh-DLG1-AS1+miR-497 inhibitor (anti-miR-497). (C) Compared with adjacent normal tissues, the expression of YAP1 increased in PTC tissues. (D) Quantitative real-time polymerase chain reaction (qPCR) assays showing upregulated expression of YAP1 in four PTC cell lines as compared with that in a normal thyroid epithelial cell line (Nthy-ori 3-1). (E) Analysis of correlation between YAP1 and miR-497 expression in PTC tissues by Pearson’s correlation analysis. (F) Analysis of correlation between YAP1 expression and DLG1-AS1 expression in PTC tissues by Pearson’s correlation analysis. All experiments were performed in triplicate, data are expressed as mean ± standard deviation (SD) (*P < 0.05, **P < 0.01).