Research Paper Volume 12, Issue 20 pp 20024—20046

GDF11 induces mild hepatic fibrosis independent of metabolic health

Figure 3. GDF11 induces HSCs activation in mice. 16-18 months old wild type C57/BL6J mice (n=5 mice per group) were injected daily (for nine days) with either GDF11 (1 mg/kg) or saline. (A) Representative images of H&E stained livers (200x magnification) from the livers of CTL and GDF11-treated mice. (B) Quantitative morphometric analysis of total lipid area (%) as in (A). (C) Representative images of αSMA immunostaining (200x magnification) in the livers of CTL and GDF11-treated mice. (D) Quantitative morphometric analysis of αSMA immunostaining (% of total imaged area, n=5 per group, at least fifteen randomly chosen fields per sample were evaluated). Data are represented as the mean ± SD. ** p<0.01 (Mann-Whitney U-test).